Integrated DNA Technologies
PrimeTime® qPCR Assays
PrimeTime® qPCR probe-based assays—the gold standard for quantitative gene expression studies.
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Select from various combinations of dyes and quenchers
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Choose from 3 reactions scales— 100, 500 or 2500 reactions
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Order in tubes or 96-well plates
PrimeTime qPCR Primer Assays are provided without probe for intercalating dye experiments.
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Receive 5 nmoles each of forward and reverse primers mixed, sufficient for 500 reactions (20 µL)
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Order in tubes or 96-well plates
PrimeTime® qPCR Probes
PrimeTime qPCR DNA probes are non-extendable oligonucleotides labeled with a 5’ fluorescent reporter and a 3’ quencher dye licensed for use in 5’ nuclease qPCR assays (with companion dye and quencher licenses).
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Traditional dual-labeled probes are available with a wide variety of dyes and quenchers.
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Choose IDT proprietary ZEN™ Double-Quenched Probes for superior performance compared to traditional dual-labeled probes.
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Ultra-small-scale Mini Probes delivered at 0.5 nmole allow researchers to conduct initial screening at low cost.
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Eco Probes are delivered at 2.5 nmoles normalized yield, sufficient for ~500 reactions for gene expression analysis.
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Express Probes are ready for shipment in one business day.
Custom qPCR Probes
Generate reliable, high-quality gene expression analysis data for a broad range of sample types with our double- and single-quenched PrimeTime® qPCR Probes.